Application

Western blot: 1:1,000-1:5,000 using ECL.
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 µg/mL

Research Sub CategoryCytoskeleton

This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.

Epitope: phospho-specific (Ser19)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.

Specificity

Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.